Supplementary Figure 8: YJR012C and UTR5 are not essential
From: Highly parallel genome variant engineering with CRISPR–Cas9
a, RNA-seq and ribosome footprinting read depth by position (Albert, F. W., Muzzey, D., Weissman, J. S. & Kruglyak, L. Genetic influences on translation in yeast. PLoS Genet. 10, e1004692; 2014) in the vicinity of YJR012C and GPI14. We mark the position of YJR012C(M76), which we propose to be the actual start of YJR012C. b, Alignment of the annotated YJR012C protein sequence from related yeast species. The proposed start position at M76 is highlighted with a red box. The 48 codons of S. mikatae YJR012C not shown include two additional stop codons. c, Tetrad dissections of a yjr012c(76-207Δ)/YJR012C diploid strain, as in Fig. 2. n = 12 tetrads were analyzed. d The positions of UTR5, HYP2, and the TATA box of HYP2 (left) (Rhee, H. S. & Pugh, B. F. Genome-wide structure and organization of eukaryotic pre-initiation complexes. Nature 483, 295–301; 2012). Tetrad dissections of a utr5(34-166Δ)/UTR5 diploid strain; n = 4 tetrads were analyzed (right).
