Supplementary Figure 9: MMF1 is conditionally essential
From: Highly parallel genome variant engineering with CRISPR–Cas9
Tetrad dissections of an mmf1Δ/MMF1 strain were done as in Fig. 2, with pictures taken after four days of growth. Top two panels show tetrads dissected on rich medium; bottom two panels show tetrads dissected on defined (CSM) medium. Left two panels show tetrads dissected on 2% glucose medium; right two panels show tetrads dissected on 2% galactose medium. The original annotation of essential genes was done in rich glucose medium (top left), whereas our PTC tolerance experiment was done in defined galactose medium (bottom right). n = 6 tetrads were analyzed for each condition tested.
