Supplementary Figure 11: Predicted effects of human NMD on PTCs, for human genes that are homologs of essential yeast genes
From: Highly parallel genome variant engineering with CRISPR–Cas9

For human genes, we plot the fraction of PTCs escaping NMD regulation according to the 50-bp rule (Nagy, E. & Maquat, L. E. A rule for termination-codon position within intron-containing genes: when nonsense affects RNA abundance. Trends Biochem. Sci. 23, 198–199; 1998) versus distance from gene ends. Note that the majority of PTCs within the last 27 codons are predicted to escape NMD regulation.