Supplementary Figure 12: Modifications to gRNA sequence to enable bulk cloning
From: Highly parallel genome variant engineering with CRISPR–Cas9
Sequence numbering corresponds to the nucleotide position in the gRNA sequence. (a) The end of the SNR52 promoter sequence and start of the gRNA. The upper sequence is what was used for S. cerevisiae sgRNA expression by DiCarlo, et al. (2); the lower sequence shows the modifications, in red, that incorporated a BstEII cloning site. (b) The first gRNA hairpin. The left sequence is what DiCarlo, et al., used; the right sequence shows the modifications, in red, that incorporated an SphI cloning site.
