Supplementary Figure 14: Histogram of barcoded-plasmid persistence
From: Highly parallel genome variant engineering with CRISPR–Cas9
We fit a generalized linear model to the read count data for each tracked barcoded plasmid. This histogram shows the resulting slopes (thetas) for the barcoded plasmids. The vertical red line demarcates our “persisting” versus “depleted” binarization threshold of −0.025.
