Supplementary Figure 4: PTCs were similarly deleterious in nej1∆ nmd2∆ and nej1∆ NMD2 cells

a, Tolerance score for each tested PTC in essential genes and dubious ORFs in nej1∆ nmd2∆ cells, with overlaid boxplots (n = 8,346 PTCs in essential genes and 695 PTCs in dubious ORFs). The centerline of each box corresponds to the data's median value; the top and bottom of the box span from the first quartile to the third quartile of the data; and the whiskers reach to either the data's most extreme values or 1.5 times the interquartile range. P < 2 × 10⁻¹⁶, two-sided Wilcoxon rank test. b, Scatterplot of PTC tolerance score versus distance in codons from the 3′ ends of essential genes in nej1∆ nmd2∆ cells (top) and in nej1∆ NMD2 cells (bottom; same as Fig. 1d). As in Fig. 1d, the thick blue line shows a two-segment regression fit, and the 95% confidence interval for the boundary between the segments is shown by the vertical blue lines. The segmented regression was fit on PTC tolerance scores for n = 7,583 PTCs and n = 7,561 PTCs that were within 500 codons of the 3′ end of a gene, for the top and bottom panels, respectively.