Supplementary Figure 6: Tolerance of end truncation
From: Highly parallel genome variant engineering with CRISPR–Cas9
a, Histogram of the number of genes tolerating a given number of tested PTCs. For each gene, the number of tolerated PTCs was determined by a Hidden Markov Model analysis. b, The 3′-most tested PTC in POB3, at position 551, had a tolerance score of -1.2. It was also called deleterious by the HMM analysis. We confirmed that POB3 does not tolerate a deletion spanning its terminal two codons (n = 6 tetrads analyzed), and found that it tolerates deletion of its last codon (n = 8 tetrads analyzed). Tetrad dissections were done as in Fig. 2, from a diploid yeast heterozygous for a truncation mutation of interest. c, The 3′-most tested PTC in PCNA, at position 251, had a tolerance score of -0.59, and was called deleterious by the HMM analysis. We confirmed that PCNA does not tolerate a deletion spanning its terminal eight codons, and discovered that terminal deletions of five or more codons were lethal. n = 6 tetrads were analyzed for each truncation mutant.
