Supplementary Figure 7: Relationship of TPMT variant abundance to drug sensitivity.
From: Multiplex assessment of protein variant abundance by massively parallel sequencing
a, Scatterplot comparing abundance scores and previously characterized red blood cell (RBC) activity from patients. b, c, Scatterplots comparing individually assessed, WT-normalized EGFP:mCherry geometric means to previously published values of average RBC activity (b), or average patient dosage intensity (c). Dose intensity is the dose where 6-MP becomes toxic to the patient before reaching the 100% protocol dose of 75 mg/m2. r and ρ denote Pearson’s and Spearman’s correlation coefficients, respectively. n = 6 samples for each plot. d, Western blotting results for individually-expressed TPMT variant GFP fusions. Each variant was blotted with 45, 15, and 5 µg of total protein input per lane. This experiment was performed once
