Supplementary Figure 10: Scheme to determine total frequency filtering threshold value.
From: Multiplex assessment of protein variant abundance by massively parallel sequencing
a, b, Scatterplots showing the total frequencies and weighted average values of wt (black), synonymous variants (red), or non-terminal nonsense variants (blue) for each experiment, for PTEN and TPMT respectively. A combination of synonymous variant coefficient of variation (c and d), synonymous variant mean (black) and median (red) (e and f), and total number of scored missense variants (g and h) for PTEN (c, e, and g) and TPMT (d, f, and h) were assessed at increasing total frequency filtering threshold values to obtain the threshold value that we required across the four bins for a variant to be included in the analyses we present. The 1 x 10-4.75 total frequency threshold used for the final analysis is displayed as a dotted line in each plot
