Supplementary Figure 1: Correlation of ATAC–seq peaks between eye–antennal discs (DGRP) and adult brain tissues.

Spearman correlation between the accessible regions (normalized ATAC–seq reads) of 30 eye–antennal disc samples (DGRP) and 2 adult brain samples. a, Pairwise correlation matrix of all 32 samples. b–e, Correlation plots visualizing normalized (RPM) ATAC–seq reads for all 34,768 accessible regions (over the 30 EA discs and 2 brain samples). b, Correlation (Spearman’s ρ = 0.95) between irradiated and control tissue. c, Correlation (Spearman’s ρ = 0.90) between different DGRP lines. d, Correlation (Spearman’s ρ = 0.96) between the two adult brain samples. e, Low correlation (Spearman’s ρ = 0.27) between the accessible regions of eye–antennal discs (DGRP 25,199) and adult brain tissues.