Supplementary Fig. 11: Generation of recombinant αN-catenin protein. | Nature Genetics

Supplementary Fig. 11: Generation of recombinant αN-catenin protein.

From: Biallelic loss of human CTNNA2, encoding αN-catenin, leads to ARP2/3 complex overactivity and disordered cortical neuronal migration

Supplementary Fig. 11: Generation of recombinant αN-catenin protein.

GST-tagged full-length αN-catenin, and a fragment of αN-catenin containing the actin-binding domain (ABD), was produced in BL21 bacterial cells. Cell lysates were run on an SDS–PAGE gel alongside BSA standards and Coomassie stained to show full-length and ABD αN-catenin expression (black arrows) following affinity purification. Repeated in triplicate.

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