Supplementary Fig. 13: The actin-binding domain of αN-catenin is sufficient to repress Arp2/3-mediated actin polymerization.

a, Full-length western blots corresponding to the cropped western blots in Fig. 4a. Repeated in three biological replicates. b, Full-length western blots corresponding to the cropped western blots in Fig. 4c. Repeated in two biological replicates. c, Actin polymerization assay showing dose-dependent inhibition of Arp2/3 + VCA domain of WASP mediated actin polymerization by recombinant αN-catenin ABD, measured by relative fluorescent units. In the absence of Arp2/3 (black), there was minimal polymerization, but when Arp2/3 + VCA was added (green) polymerization increased substantially, the effect of which was reversed upon dose escalation of αN-catenin ABD. Repeated in duplicate.