Supplementary Figure 1: FISH reveals eve enhancer-dependent expression of a reporter gene located 142 kb upstream of the endogenous eve locus.
From: Dynamic interplay between enhancer–promoter topology and gene activity
a, Genomic design of a synthetic long-range enhancer–promoter interaction. An ectopic homie insulator sequence with an eve promoter driving lacZ is integrated at ~142 kb upstream of the eve locus. Embryos homozygous for this construct are hybridized with single-molecule FISH probes to label endogenous eve (red) and lacZ (green) mRNA. b, Top, surface view of a 2.5-h-old Drosophila embryo hybridized with eve-atto633 probes. Anterior is to the left. Bottom, z-stack projection of the marked region in the top panel. LacZ activity (labeled with lacZ-atto565 probes) only occurs sporadically within the limits of the eve pattern (red). This lacZ pattern appears in all 13 embryos imaged (2–3 h old) and a representative sample is shown here.
