Supplementary Figure 5: Sustained physical proximity is required for transcription initiation and maintenance: individual traces.
From: Dynamic interplay between enhancer–promoter topology and gene activity
a, Transcriptional activity (red spot (PP7) intensity) and instantaneous enhancer–promoter distance (blue-green distance) as a function of time for 286 nuclei transitioning from the Red-OFF to the Red-ON state. Time series for individual nuclei are aligned such that PP7 activity starts at 0 min (red dashed lines). Individual traces are sorted according to the mean enhancer–promoter distance in the 5 min before PP7 activity is observed. b, Distribution of the instantaneous enhancer–promoter distance as a function of time for the Red-OFF to Red-ON transition. Calculated from a. c, Transcription and instantaneous enhancer–promoter distance as a function of time for 203 nuclei transitioning from the Red-ON to the Red-OFF state. Time series for individual nuclei are aligned such that PP7 activity ends at 0 min (red dashed lines). Individual traces are sorted according to the mean enhancer–promoter distance in the 5 min before PP7 activity disappears. d, Distribution of the instantaneous enhancer–promoter distance as a function of time for the Red-ON to Red-OFF transition. Calculated from c.
