Supplementary Figure 1: Suppression of endogenous wild-type but not mutant TP53 impacts cellular fitness in human cancer cells.
From: Mutational processes shape the landscape of TP53 mutations in human cancer
a,b, Comparison of enrichment scores (Cell 170, 564–576, 2017) for RNAi reagents targeting TP53 (a) or MDM2 (b) in cell lines with differing p53 status. The functional and genetic p53 status of each cell line was defined using publicly available p53 target gene expression, nutlin-3 sensitivity, and TP53 sequencing data (Supplementary Table 1). Only cell lines with concordant functional and genetic classifications were included in the analyses in a–c. Each point represents the gene-level score for a given cell line, and error bars indicate the mean and s.d. of each group. c, p53 protein expression in cancer cell lines as measured by reverse-phase protein array (RPPA). d–g, Comparison of enrichment scores for TP53-targeting reagents for cell lines with concordant (d,e) or discordant (f,g) genetic and functional TP53 status (*P < 0.05, ****P < 0.0001, two-tailed Welch’s t-test).
