Supplementary Figure 7: FORGE DNase I–sensitive region enrichment in known sentinel SNPs, compared to known and novel sentinel SNP associations for blood pressure.

Sentinel SNPs were investigated for enrichment in ENCODE DNase I regulatory regions using FORGE. The background probability of overlap is determined from the 1,000 background set overlap counts and the probability of the observed test result under a binomial distribution is calculated. The P-value thresholds of 0.05 and 0.01 are corrected for multiple testing by division by the number of tissue groupings tested, and the corrected threshold is used. Strongest enrichment in known SNPs was seen in vasculature (Human Aortic artery fibroblast (AoAF) and also Human Villous Mesenchymal Fibroblasts (HVMF) found in placenta). Enrichment in all known and novel SNPs was increased across vasculature (AoAF; HMVEC, Human microvascular endothelial cells) and highly vascularized tissues. Tissues in red are significant after correction for false discovery.