Supplementary Figure 8: Germination variation of the wild-type and transgenic lines with different genotypes.
From: Parallel selection on a dormancy gene during domestication of crops from multiple families
a, Germination of the cultivated rice HJ19 and transgenic lines. Transgenic lines were generated by introducing a genomic sequence from O. rufipogon (IRGC 105491) into HJ19. Means ± s.e.m. are shown, n = 3 independent experiments. Scale bar, 5.0 mm. b, Sequencing of PCR products containing targeted sites in OsgCR T2 plants. The red arrow indicates the deleted sequence. c, Relative expression of OsG in ZH11, CRISPR-Cas9 genome-edited (OsgCR), and overexpression (OE) lines. d–f, Germination of ZH11, OsgCR, and overexpression (OE) lines. Osg-OE and OsG-OE lines were generated by introducing DNA coding sequences from ZH11 and O. rufipogon (IRGC 105491), respectively, into ZH11. Freshly harvested spikes (d,e) and spikes stored for 6 months (f) were used in the experiments. Means ± s.e.m. are shown, n = 3 independent experiments. Scale bar, 5.0 mm.
