Supplementary Fig. 7: Variation at RH-seq hit loci has little impact on growth at 28 °C in the background of the interspecific hybrid.

Each panel reports growth efficiency measurements of targeted deletion reciprocal hemizygotes at the indicated RH-seq hit locus. In a given panel, the left-hand pair of bars reports the relative efficiencies of targeted deletion hemizygotes after culture at 39 °C, from Fig. 2b. In the right-hand pair of bars, each bar reports the mean growth efficiency (n = 12–36 cultures) after culture at 28 °C of a targeted deletion hemizygote in the indicated species’ allele, normalized by the analogous quantity for the wild-type hybrid parent; individual measurements are reported as circles. Statistical analyses of 39 °C efficiency data are reported in Fig. 2; *P ≤ 0.05, in a two-sample, two-tailed t test for a difference in efficiency between the indicated hemizygotes at 28 °C. See Supplementary Table 1 for exact P values and sample numbers.