Supplementary Figure 3: mRNA expression in myeloid cells of genes discovered to be phagocytosis screen hits and correlation of replicate screens.

a, mRNA expression levels of genes passing 10% FDR for each substrate in differentiated U937 cells and primary human microglia. Plots illustrate mean (bar), minimum, and maximum values, with each gene’s mean FPKM value derived from n = 4 sequenced replicates. b, Correlation of replicate genome-wide screens. Signed confidence score (casTLE score) of each genome-wide screen plotted against a biological replicate screen. The signed confidence score is negative if gene knockout inhibits phagocytosis and positive if it promotes phagocytosis. Each casTLE score is derived from n = 2 replicate screens. c, Correlation of replicate-focused batch retest screens. The signed confidence score (casTLE score) of each focused batch retest screen is plotted against a biological replicate screen. The signed confidence score is negative if gene knockout inhibits phagocytosis and positive if it promotes phagocytosis. Each casTLE score is derived from n = 2 replicate screens.