Supplementary Fig. 1: Phenotypes of zmynd10 mutants. | Nature Genetics

Supplementary Fig. 1: Phenotypes of zmynd10 mutants.

From: Cilia-driven cerebrospinal fluid flow directs expression of urotensin neuropeptides to straighten the vertebrate body axis

Supplementary Fig. 1: Phenotypes of zmynd10 mutants.

a,b,b′, External phenotypes of wild-type zebrafish and mutants resulting from two different zmynd10 mutations (del7 and del10) at 72 h.p.f. c,c′,c′′, Ventral views of heart looping phenotypes in control and zmynd10 mutants. Control embryos display normal heart looping (c), while heart looping is frequently inverted (c′) or absent (c′′) in zmynd10 mutants, implying defective cilia motility in the left–right organizer. a, atrium; v, ventricle. d, Protein and genomic structures of the zmynd10 gene. The blue bar indicates the MYND-type zinc-finger domain. The bottom panel shows sequences of the zmynd10 gene in wild-type and mutant alleles. Red color shows TALEN spacer sequence. e, Lateral views of the otic vesicle showing two otoliths in wild-type embryos (e) and multiple otoliths in zmynd10 mutants (e′), implying defective motility of cilia in the otic vesicle. f, Histological analysis showing a distended pronephric tubule lumen in zmynd10 mutants (f′, asterisk; implying defective motility of pronephric duct cilia), compared with wild-type control embryos (f). Scale bars: a,b, 500 µm; e,f, 50 µm.

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