Supplementary Figure 8: The epigenetic landscape at the TFAP2C locus.

a, TFAP2C regulation is both morphogen and p63 dependent, n = 2. b, At the TFAP2C locus, within 400 kb, there is one p63 binding site, one morphogen- and p63-dependent ATAC-seq peak, and one p63-dependent H3K27me3 region (raw signal visualized by IGV tracks, n = 2). Cohesin HiChIP shows that these elements are highly connected upon morphogen treatment and p63 presence (n = 3). The four virtual 4C plots represent interactions from four viewpoints within this locus, and these viewpoints are labeled in the top left corner of each graph and highlighted in gray. c, Schematics to represent the different chromatin connections present at the TFAP2C locus in all four cellular contexts. d, The ATAC day 7 peak was deleted using CRISPR–Cas9 to generate a heterozygous knockout (ATAC-KO) for the region downstream of TFAP2C and to determine the effects of its loss on TFAP2C expression. Immunofluorescence analysis reveals that loss of the ATAC day 7 peak results in a significant decrease in TFAP2C expression at day 7 (*P = 0.04 from three independent images). Error bars represent s.e.m. Scale bar, 20 μm.