Supplementary Figure 9: UMI-4C validation of the cohesin HiChIP.

a, Virtual 4C comparison of the UMI-4C data and the cohesin HiChIP data in the day 7 p63WT and day 7 p63KO cell lines. The left half of the panel depicts the UMI-4C with the following three primer viewpoints (indicated by the dotted line): the TSS, the p63 binding site, and the ATAC day 7 peak at the TFAP2C locus. The data were normalized to the number of valid reads per locus and the gray shading represents s.e.m. from NextSeq replicates (n = 2). Sequencing depth was comparable between the two cell types displayed. The right panel depicts the Virtual 4C plots of HiChIP and shows similar long-range interactions to those detected using UMI-4C. b, UMI-4C viewpoint primers used to detect long-range interactions. All DS primers were synthesized with the Illumina prefix listed and the universal primer was used in both rounds of PCR as the reverse primer. TruSeq-indexed adaptors 3–6 were used in this study.