Supplementary Figure 3: SCHLAP1 does not affect SWI/SNF composition or localization.
From: SWI/SNF remains localized to chromatin in the presence of SCHLAP1
a, Salt fractionation of RWPE1;LACZ and RWPE1;SCHLAP1 cells for SWI/SNF subunits from whole cells. The experiment was repeated twice with similar results. C, cytosol; N, nucleus; M, micrococcal nuclease digestion. Numbers denote salt fraction (mM). b, Immunofluorescence of RWPE1;LACZ or RWPE1;SCHLAP1 cells for SMARCB1 or SMARCA4. The experiment was repeated twice with similar results. c, Co-immunoprecipitation for SMARCA4 and western blot for other SWI/SNF subunits. The experiment was repeated twice with similar results.
