Fig. 3: Identification of favorable chromosome segments controlling fiber quality by using introgression lines.
a, Construction of an introgression line population by using G. hirsutum Emain22 (as a recurrent parent) and G. barbadense 3–79 (as a donor parent). The upper track shows the fiber characteristics of both cotton accessions. The lower track shows the distribution of introgression segments identified from the 168 introgression lines along the 26 chromosomes in G. hirsutum. The x-axis shows the 26 chromosomes and the y-axis shows the 168 introgression lines. b, Fiber characteristics for introgression line N29. The right circle shows the introgression segment in the chromosome D12 (from 47.4 Mb to 54.6 Mb) which is indicated by the red box. c, Mapping-by-sequencing of Xuzhou142fl. Ratio of SNPs in chromosomes A12 and D12 between two different pools from the F2 population (one pool consisting of cotton plants with the same fiber characteristics of Xuzhou142 and one pool consisting of cotton plants exhibiting the same phenotype with Xuzhou142fl). d, Distribution of fiber-quality related quantitative trait loci (QTLs) in chromosomes of G. hirsutum. Each QTL is indicated by a red box. The specific location of each QTL is shown in Supplementary Table 30. e, QTL mapping for the trait of fiber length (mm). f, QTL mapping for the trait of fiber elongation rate (%). For e and f, the x-axes show all the introgression segments and the y-axes show the logarithm of odds (LOD) score. The physical locations of QTLs are shown with arrows.
