Supplementary Figure 7: Frequency and types of segregation errors of clones of tumor PDO 16T are stable over time.
From: Ongoing chromosomal instability and karyotype evolution in human colorectal cancer organoids
(a) Quantification of segregation errors per organoid observed in tumor PDO 16T clones at three time points (3, 15, 24 weeks after generation of the monoclonal line). n= 1 independent experiment. The data is represented as a box-and-whisker plot with the location of the whiskers based on the Tukey method. The boxes represent quartiles 2 and 3. The horizontal line in the box represents the median. The whiskers represent the lowest values within 1.5 times the interquartile range of the lower quartile (Q1-1.5*(Q3-Q1)), and the highest values within 1.5 times the interquartile range of the upper quartile (Q3+1.5*(Q3-Q1)). The circles are the data points which are more than 1.5 times the interquartile range from the end of a box. (b) Quantification of types of segregation errors. Graph shows the mean segregation error per organoid. (c) Heterogeneity and aneuploidy scores in tumor PDO 16T clones at two time points (weeks 3 and 24) as determined by scKaryo-Seq.
