Supplementary Fig. 2: Mapping of gene trap integrations and validation of MTHFD1.
From: MTHFD1 interaction with BRD4 links folate metabolism to transcriptional regulation
a, Representation of the gene-trap integration sites mapped on MDC1 and MTHFD1 genes. Red arrows indicate sense insertions; blue arrows indicate antisense insertions. b, Western Blot for MTHFD1 protein levels after downregulation with the indicated shRNAs in REDS3. Tubulin was used as loading control. The experiment was repeated twice with similar results. c, Validation of MTHFD1 in an alternative REDS clone described previously (Sdelci, S. et al. Mapping the chemical chromatin reactivation landscape identifies BRD4-TAF1 cross-talk. Nat Chem Biol 12, 504-10 (2016)). Quantification of RFP positive cells from live-cell imaging pictures of REDS3 cells treated with MTHFD1 shRNA. Two biological replicates were done for each experimental condition. d, Representative live-cell images of MTHFD1 knock down in REDS3 cells. RFP signal is shown in red; scale bar 100 μm.
