Supplementary Figure 1: Gradual fusion upon progressive CTCF site deletion. | Nature Genetics

Supplementary Figure 1: Gradual fusion upon progressive CTCF site deletion.

From: Functional dissection of the Sox9–Kcnj2 locus identifies nonessential and instructive roles of TAD architecture

Supplementary Figure 1: Gradual fusion upon progressive CTCF site deletion.The alternative text for this image may have been generated using AI.

(A) cHiC chromatin interactions from wildtype and mutant E12.5 mouse embryonic limb buds (n=1). CTCF ChIP-seq with binding site orientation (red/blue) at the boundary and intra-TAD are highlighted. Two-headed arrow indicates two significant motifs (FIMO, p<10-4) underlying the ChIP-seq peak. Virtual 4C from Kcnj2 (blue) and Sox9 (orange) below each map. Progressive deletion of CTCF sites causes progressively increasing interaction between Sox9- and Kcnj-TADs (B) Subtraction maps for the same region show increases (red) and decreases (blue) in interactions upon deletion of additional intra-TAD CTCF-sites.

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