Extended Data Fig. 8: WNT3a represses MYC expression in HCEC cells.
Primary cultures of colon epithelial cells were treated with WNT3a at different concentrations and for different lengths of time, as indicated in the image. Total RNA was extracted from two independent experiments and MYC mRNA expression levels were examined by qRT-PCR using primers for exon2, as described in the Methods section. The results were normalised to total cDNA input. Ctrl = control cells.
