Extended Data Fig. 2: ARID1A co-binds ER and FOXA1-bound regulatory elements, but is depleted with estrogen treatment. | Nature Genetics

Extended Data Fig. 2: ARID1A co-binds ER and FOXA1-bound regulatory elements, but is depleted with estrogen treatment.

From: ARID1A influences HDAC1/BRD4 activity, intrinsic proliferative capacity and breast cancer treatment response

Extended Data Fig. 2: ARID1A co-binds ER and FOXA1-bound regulatory elements, but is depleted with estrogen treatment.

a-c. Single gene profiles showing the binding of ER, FOXA1 and ARID1A on overlapping sites in MCF7 cells. ChIP-seq was performed using three independent biological cell cultures. d. Overlap of binding sites for ER, FOXA1 and ARID1A binding sites in ZR-75-1 cells. e. Boxplots showing the normalized ChIP-seq tag density around 400 bp window around the center of ARID1A binding on DiffBind-defined estrogen independent (constant) and dependent (reduced with estrogen) sites in MCF7. Both classes show reduced ARID1A binding upon estrogen. p-values were calculated by Welch’s t-test, two-sided. Centre line shows the median values with bounds of box corresponding to the first and third quartiles and the upper and lower whiskers extend to the largest or the smallest value no further than 1.5 × IQR (inter-quartile range). Statistical test details are mentioned in Supplementary Table 5e.

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