Extended Data Fig. 8: Transiently overexpressed ACE2-GFP and dACE2-GFP are detectable on the cell surface. | Nature Genetics

Extended Data Fig. 8: Transiently overexpressed ACE2-GFP and dACE2-GFP are detectable on the cell surface.

From: Interferons and viruses induce a novel truncated ACE2 isoform and not the full-length SARS-CoV-2 receptor

Extended Data Fig. 8

T24 cells were transiently transfected for 24 hrs with indicated constructs or not transfected (control) and then treated with (+) or without (-) biotin. Western blots with the C-terminal anti-ACE2 antibody detected a similar expression of both proteins in input lysates, but only in the biotin+ fraction after pull-down with streptavidin beads, showing much stronger expression of ACE2 compared to dACE2. GAPDH, a cytoplasmic protein, is undetectable in the pull-down fraction. The experiment was repeated three times, and representative results are shown.

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