Extended Data Fig. 5: ISG expression in THP-1 cells after LSM11 knockdown.

a, Expression of interferon beta and selected interferon stimulated genes (ISGs) following 4 to 5 days LSM11 lentivirus shRNA transduction of THP-1 cells. b,c, Expression of interferon beta and selected ISGs (b) and of ISG15 protein (c) following LSM11 lentivirus shRNA transduction of THP-1 cells wild-type or null for cGAS, STING or MAVS. Cofilin was used as a housekeeping protein. The white asterisk indicates a non-specific band. Box plots show the minimum and maximum scores (bottom and top whisker, respectively) outside the interquartile range, the lower quartile, the median, and the upper quartile. Data points in the box plots represent the value for each experiment. Differences in expression within groups were assessed by Kruskal-Wallis H test, followed by Dunn’s multiple comparison test. Error bars indicate standard deviation. Experiments were performed with a minimum of 3 technical replicates.