Extended Data Fig. 2: LSM11 knockdown and RDH pre-mRNA expression in fibroblasts.
From: cGAS-mediated induction of type I interferon due to inborn errors of histone pre-mRNA processing
a,b, The effect of knockdown of LSM11 in HCT116 (a) and U2OS (b) cells on the processing of replication-dependent histone mRNAs. Primers surrounding the cleavage site were used to assess the presence of polyadenylated RDH transcripts after 24 h knockdown of LSM11 by siRNA. Error bars indicate standard deviation from the mean. Two-tailed Mann-Whitney U test was used to compare differences between two groups. Experiments were performed with a minimum of 3 technical replicates. c, Misprocessing of RDH mRNAs in fibroblasts. Bar charts showing the expression of polyadenylated (Poly(A)+) and non-polyadenylated (Poly(A)-) replication dependent linker (H1) or core (H2A, H2B, H3, H4) histone transcripts in control (n = 3) and AGS8/9 patient-derived (n = 3) fibroblasts.
