Extended Data Fig. 8: Percent of targets from the priming screen that validate.
From: Genetic screening for single-cell variability modulators driving therapy resistance
To assess the sensitivity of our screen, we validated the effect observed in the initial priming screen for a select group of targets via NGFR immunofluorescence. Here, each dot represents an individual single guide RNA, and we plot the change in single guide RNA representation between NGFRHIGH/EGFRHIGH cells and controls (as measured in the priming screen). We then organize all sgRNAs into tiers (y-axis, Tiers 1 through 4) based on the percent of single guide RNAs against a target showing at least a two-fold change in representation on NGFRHIGH/EGFRHIGH cells. In red, we labeled targets that when tested again produced at least a 50% change in the frequency of NGFRHIGH cells. In black, we labeled targets that we tested but did not validate, and in gray we show targets we did not test. We display the percent of genes tested and validated at each tier on the right.
