Extended Data Fig. 7: Lm, Lp and Ws values in tumors simulated under monoclonal versus polyclonal seeding.
The number of sSNVs in each of the three categories (M-private clonal or Lm, P-private clonal or Lp, P/M shared subclonal or Ws) in the simulated data generated by modeling monoclonal seeding or polyclonal seeding within an agent-based model (Methods) where one sample (~106 cells) was biopsied from each primary tumor and metastasis. We employed a mutation rate u = 0.6 per cell division in exonic regions (corresponding to 10-8 per site per cell division in the 60 Mb diploid coding regions). In order to account for varying scenarios of tumor growth dynamics, selection and timing of metastatic dissemination, the birth probability b of founding cells, selection coefficient s and primary tumor size at dissemination Nd was randomly sampled from a uniform distribution, b~U(0.55, 0.65), log10(s)~U(-3,-1) and log10(Nd)~U(4,8), respectively. A total of n = 500 virtual P/M pairs were simulated under monoclonal seeding and polyclonal seeding by randomly sampling these three parameters. Bar, median; box, 25th to 75th percentile (interquartile range, IQR); vertical line, data within 1.5 times the IQR.
