Extended Data Fig. 5: YBX3 mediates translation efficiency of m6A modified transcripts.
a, Sucrose gradient A260 absorbance profile from YBX3 knockdown and control Hela cells. The arrows indicate the fraction sampled for subsequent qPCR analysis of YBX3 target transcripts. This experiment is repeated 2 times. b, Translation efficiency of YBX3 targets in comparison with YTHDF1 targets. We accounted for mRNA level variation by dividing polysome-bound fraction by the non-polysome-bound fraction. Transcript levels are quantified using RT-qPCR. Three polysome-bound fractions, as indicated in panel a, are sampled from sucrose gradient fractionation. 2 technical replicates were measured to obtain the data. The lower and upper hinges correspond to the first and third quartiles. Horizontal line indicates median value, and whiskers correspond to the value no further than 1.5x inter-quartile range.
