Extended Data Fig. 9: CUL3 KO cells have a unique transcriptomic signature. | Nature Genetics

Extended Data Fig. 9: CUL3 KO cells have a unique transcriptomic signature.

From: Characterizing the molecular regulation of inhibitory immune checkpoints with multimodal single-cell screens

Extended Data Fig. 9: CUL3 KO cells have a unique transcriptomic signature.

a, Single-cell mRNA expression heatmap showing that CUL3 KO cells upregulate a module of genes in comparison to NT and CUL3 NP cells (including the PD-L1 transcript (CD274), highlighted on the heatmap). b, Single-cell mRNA expression heatmap showing that the CUL3 transcriptomic signature is not IFNγ-related, suggesting CUL3 is acting through an alternative pathway to regulate PD-L1 at the transcriptional level. For both (b) and (c) heatmaps, lists of genes were obtained using FindMarkers() function in Seurat (Wilcoxon Rank sum test). mRNA counts are log-normalized and scaled (z-score).

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