Extended Data Fig. 7: Phenotypic and functional analysis of Patient 1T cells.

a, Summary of CD3⁺CD8⁺ T cell frequencies of adult healthy donors (HD) (HD adult: n = 26) and 9 months to 14 years old HD (HD 9 months to 14 years: n = 17) and of Patient 1 at 1.5 years and 2 years of age (n = 2, each 3 technical replicates) expressed as percent of live CD3⁺ T cells. b, Summary of CD3⁺CD8⁺CD25^– memory T cell frequencies as assessed by surface staining of CD45RA and CCR7. c,d, Summary of IFN-γ- (c) and TNF-producing (d) CD3⁺CD8⁺CD25^– memory T cell frequencies as assessed by intracellular cytokine staining following 5 hours PMA (0.2 μM) and Ionomycin (1 μg/mL) stimulation. e, Summary of CD3⁺CD4⁺CD25^– memory T cell frequencies as assessed by surface staining of CD45RA and CCR7. f, Summary of CD3⁺CD4⁺CD25^–CCR6⁺ memory T cell frequencies as assessed by surface staining. g, Summary of CD3⁺CD4⁺CD25^–RORγt⁺ memory T cell frequencies as assessed by combined surface and intracellular staining. h,i,j, Summary of TNF- (h), IL-10- (i) and IL-13-producing (j) CD3⁺CD4⁺CD25^– memory T cell frequencies as assessed by intracellular cytokine staining following 5 hours PMA (0.2 μM) and Ionomycin (1 μg/mL) stimulation. k, CCR6⁺CCR4⁺CXCR3^– (Th17-enriched) and CCR6⁺CXCR3⁺ CCR4^– (Th1/Th17-enriched) CD3⁺CD4⁺CD25^– memory T cell frequencies as assessed by surface staining and flow cytometry.