Extended Data Fig. 5: SDS-PAGE analysis of seed storage proteins extracted from the mature grains of Weining rye. | Nature Genetics

Extended Data Fig. 5: SDS-PAGE analysis of seed storage proteins extracted from the mature grains of Weining rye.

From: A high-quality genome assembly highlights rye genomic characteristics and agronomically important genes

Extended Data Fig. 5: SDS-PAGE analysis of seed storage proteins extracted from the mature grains of Weining rye.The alternative text for this image may have been generated using AI.

Four types of secalins, that is, HMW-secalins, 75k γ-secalins, ω-secalins, and 40k γ-secalins, were detected. HMW-secalins and 75k γ-secalins are encoded by Sec-3 and Sec-2, respectively, whereas ω-secalins and γ-secalins are specified by Sec-1 and Sec-4. The secalins exhibited anomalous electrophoretic mobilities in SDS-PAGE because of carrying highly repetitive, proline- and glutamine-rich motifs in their proteins (Supplementary Table 19). M, protein size marker (kDa); WN SSP, Weining rye seed storage protein. The data shown were reproducible in three independent experiments.

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