Extended Data Fig. 9: More extensive crosslinking does not greatly alter Hi-C maps and chromatin interaction frequencies. | Nature Genetics

Extended Data Fig. 9: More extensive crosslinking does not greatly alter Hi-C maps and chromatin interaction frequencies.

From: Genetic and spatial organization of the unusual chromosomes of the dinoflagellate Symbiodinium microadriaticum

Extended Data Fig. 9: More extensive crosslinking does not greatly alter Hi-C maps and chromatin interaction frequencies.

a, Hi-C interaction map for chromosome 4 (Smic1.0; bin size = 50 kb; Smic1.0) for coccoid-enriched cultures fixed with formaldehyde and DSG. b, Genome-wide contact frequency P versus genomic distance s for coccoid-enriched cultures fixed with formaldehyde only, or with formaldehyde and DSG. P(s) displays three regimes (as in Fig. 4a), and the middle section of the plots have similar small exponents. Hi-C data was mapped against Smic1.0. c, Genome-wide contact frequency P versus genomic distance s for coccoid-enriched cultures fixed with formaldehyde and DSG for each of the 4 possible orientation of ligation products. Ideally all 4 orientations display identical P(s). The + - and - + orientations deviate strongly for s < 2 kb and represent Hi-C artifacts (unligated ends and self-circularized molecules respectively. Hi-C data was mapped against Smic1.0.

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