Extended Data Fig. 8: Pi accumulation inhibits AGPase production and activity.
From: A plasma membrane transporter coordinates phosphate reallocation and grain filling in cereals
a, b, Relative mRNA levels of OsAGPL2 (a) and OsAGPS2b (b) in suspension cells of NILs under various Pi treatments. Values are means ± s.d. (n = 3 biologically independent samples). P = 1.56 × 10−8 (a) and P = 1.10 × 10−5 (b) by one-way ANOVA. Different letters denote significant differences (P < 0.05) from Tukey’s HSD test. c, Measurement of AGPase activity in the WT and Ospho1;2-ko1 grains from 5 DAF to 30 DAF, values are means ± s.d. (n = 3 biologically independent samples). 5 DAF: P = 2.72 × 10−3; 7 DAF: P = 1.41 × 10−4; 10 DAF: P = 7.56 × 10−4; 15 DAF: P = 2.33 × 10−4; 20 DAF: P = 0.4068; 25 DAF: P = 0.0617; 30 DAF: P = 0.1132; **P < 0.01, ***P < 0.001, ns. not significant, by two-tailed Student’s t-test.
