Extended Data Fig. 1: Map-based cloning of Ospho1;2 for grain incomplete filling phenotype.
From: A plasma membrane transporter coordinates phosphate reallocation and grain filling in cereals
Primary mapping region a and fine mapping b of the locus to a ~5 kb region of the NIP genome with 3600 individuals from NILs population, denoted by double black headed arrowheads. This fine mapping region includes only one gene LOC_Os02g56510 that encodes OsPHO1;2. Eight recombinants delimiting the mapping region for detailed progeny analysis is presented by bars. Grey bars refer to the heterozygous allele, white and black bars refer to NIL-OsPHO1;2 and NIL-Ospho1;2 homozygous alleles, respectively. Phenotypic statistics of 100-grain weight from F3 segregation of the 8 recombinants were shown on the right. Data are means ±s.d. (n = 8 plants). *** indicates significant difference at P < 0.001 according to two-tailed Student’s t-test. 1-30-1: P = 2.88 × 10−15; 1-177-3: P = 7.10 × 10−15; 2-125-6: P = 3.31 × 10−18.
