Fig. 1: De novo assembly of the reference genome of X. tropicalis by using Hi-C and single-molecule sequencing.
From: Three-dimensional folding dynamics of the Xenopus tropicalis genome
a, Heatmap of chromosome 5 as an example to show assembly errors using the v.9.1 reference genome of X. tropicalis. b, Procedure of de novo assembly of the reference genome of X. tropicalis. c, Comparison between the v.9.1, de novo assembled and v.10.0 chromosome 5. The red lines show sequences with the orientation reversed. d, Heatmap of chromosome 5 to show that assembly errors are mostly corrected in the new version of the reference genome. e, Ideograms of X. tropicalis new reference pseudomolecules. The top track shows the positions of gaps (dark blue). Contigs longer than 1 Mb are shown in black and contigs shorter than 1 Mb are shown in light gray. The Hi-C datasets for genome assembly were generated from s9 embryos.
