Extended Data Fig. 6: Bioenergetic analyses of glycolysis following SET1B depletion. | Nature Genetics

Extended Data Fig. 6: Bioenergetic analyses of glycolysis following SET1B depletion.

From: The HIF complex recruits the histone methyltransferase SET1B to activate specific hypoxia-inducible genes

Extended Data Fig. 6

a, b, Wildtype, HIF1β depleted or SET1B depleted HeLa cells were generated as described, and incubated with or without DMOG (1 mM), Roxadustat (0.1 mM), or Daprodustat (0.1 mM) for 24 h. Cells were analyzed using a Seahorse XF analyzer by performing a Glycolytic Rate Assay. Oxygen consumption rates (OCR), extracellular acidification rates (ECAR) and the protein efflux rate derived from glycolysis (glycoPER) as a percentage of total were calculated using WAVE version 2.6.1. c, d, Bioenergetic analyses for wildtype, HIF1β depleted or SET1B depleted A549 cells, as described for HeLa cells. In A549 cells DMOG altered OCR in a HIF1β independent manner. n=3 for HeLa, n=2 for A549 for biological replicates. Representative data from one experiment shown with mean ± SD of 6 technical replicates.

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