Extended Data Fig. 1: VM-IAP methylation in F1 females is unresponsive to maternal exposure to the endocrine disruptor BPA.
From: Variably methylated retrotransposons are refractory to a range of environmental perturbations
F0 dams were fed either a control diet (7% corn oil, grey) or one of two BPA-supplemented diets two weeks prior to mating, throughout pregnancy and lactation (lower BPA dose: 10 μg/kg/day, light blue; upper BPA dose: 10 mg/kg/day, dark blue). Adult F1 female liver tissue was collected from one mouse per litter. Comparison of the average percentage of CpG methylation at 11 VM-IAPs in F1 females across exposure groups shows no significant differences (Welch’s ANOVA; n = 12, 9, and 13 females for the control diet, lower BPA dose, and upper BPA dose, respectively). Data points represent the average of the four or five most distal CpGs of the VM-IAP 5′ LTRs. Box-plot elements: centre line, median; box limits, 25th and 75th percentiles; whiskers, maximum and minimum; all data points shown.
