Extended Data Fig. 4: Immunophenotypic characterization of HSPCs isolated from bone marrow of wildtype and mutant mice. | Nature Genetics

Extended Data Fig. 4: Immunophenotypic characterization of HSPCs isolated from bone marrow of wildtype and mutant mice.

From: Mutational synergy during leukemia induction remodels chromatin accessibility, histone modifications and three-dimensional DNA topology to alter gene expression

Extended Data Fig. 4: Immunophenotypic characterization of HSPCs isolated from bone marrow of wildtype and mutant mice.

a, Representative flow cytometry plots showing proportions of hematopoietic stem and progenitors within bone marrow compartment. Lin-, lineage negative; HPC, hematopoietic progenitors (Lin−Sca1−cKit+) containing all myeloid progenitors; LSK, Lin−Sca1+cKit+; CMP, common myeloid progenitors; GMP, granulocyte/macrophage progenitors; MEP, megakaryocyte/erythroid progenitors. b, Comparison of proportions of hematopoietic stem and progenitors in their parental populations in mutant mice (Npm1flox-cA/+;Mx1-Cre, n = 9; Flt3ITD/+, n = 8; Npm1flox-cA/+;Mx1-Cre;Flt3ITD/+, n = 9) compared with wildtype mice (WT, n = 7). Student’s unpaired t-tests (two-sided) were performed for the comparisons; data are presented as mean values ± standard deviation; Only the p values < 0.05 were shown.

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