Extended Data Fig. 5: Association between DUSP4 dependency and DUSP4/6 levels. | Nature Genetics

Extended Data Fig. 5: Association between DUSP4 dependency and DUSP4/6 levels.

From: Paralog knockout profiling identifies DUSP4 and DUSP6 as a digenic dependence in MAPK pathway-driven cancers

Extended Data Fig. 5

(a) Top 5 genes correlated with BRAF CERES score (left - Pearson) or BRAF mutation (right - Point-Biserial) from the DepMap screens. (b) Scatterplot of CERES scores for BRAF and DUSP4. (c) Scatter plot of CERES score for DUSP4 compared to the expression of DUSP4 or DUSP6. (d) Scatter plot of CERES score for DUSP4 compared to the mass spectrometry-based proteomics levels of DUSP4 or DUSP6. All expression values are in log2(TPM +1). Proteomic levels are shown as normalized log2-transformed ratios to the bridge sample in each Tandem Mass Tags (TMT) 10-plex. (e) Relative viability of cells infected with sgRNAs pairs targeting DUSP4 or DUSP6. AAVS1 and chromosome 2 intergenic sgRNAs were used as controls. P-values were calculated based on linear regression analysis. Experiments in e were from three independent experiments. Data are mean ± s.e.m. *P-value ≤ 0.01, unpaired two-sided t-test for e. P-values for b-d were calculated based on linear regression analysis.

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