Extended Data Fig. 3: Characterization of synergistic paralog genes and selectivity of the PRKC paralog family in GNAQ-mutant uveal melanoma. | Nature Genetics

Extended Data Fig. 3: Characterization of synergistic paralog genes and selectivity of the PRKC paralog family in GNAQ-mutant uveal melanoma.

From: Paralog knockout profiling identifies DUSP4 and DUSP6 as a digenic dependence in MAPK pathway-driven cancers

Extended Data Fig. 3

(a) Percent of synergistic pairs in the Digenic Paralog CRISPR library with FDR below 5% across 11 cell lines. (b) Distribution of genes that exhibited synergy. (c) Distribution of paralog pairs that exhibited synergy across protein class (top) and size of paralog family (bottom). (d) Frequency of synergistic pairs observed across a varying numbers of cell lines. (e) The distribution of non-synergistic pairs and synergistic pairs by whether the genes were expressed across Cancer Cell Line Encyclopedia (CCLE) RNA-seq samples (expressed gene defined as log2(TPM+1) > 2 in at least 50% of 1270 cell lines). (f) Heatmap of GEMINI synergy FDRs for gene pairs within the PRKC paralog family. (g) Heatmap of the LFC for gene pairs within the PRKC paralog family. Results are shown for individual cell lines.

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