Extended Data Fig. 4: The absence of Cirop does not affect DFCs or midline patterning.
a-c, Analysis of zebrafish embryos injected or not with cirop mRNA by whole mount in situ hybridization (a), real-time qPCR (b) and western blotting (c). Scale bar in a, 0.1 mm. Similar results were obtained in at least 3 independent experiments with 50 embryos each. b, Data are mean ± s.e.m, ****P < 0.0001, two-tailed unpaired t-test. n = 3 biological triplicates of 30-50 embryos. c, Protein extracts from 2 independent experiments are loaded and membrane probed with an anti-cirop antibody. Similar results were obtained with a third independent experiment. d-f, Whole mount in situ hybridization in control and cirop−/− zebrafish embryos for tbxta (d), foxj1a (e), and sox17 (f) at indicated stages. Scale bars, 0.1 mm. Similar results were obtained in at least 3 independent experiments with 50 embryos each.
