Extended Data Fig. 2: Analyses of lncRNA expression variability in HEK293 and mES cells.
From: Transcriptional kinetics and molecular functions of long noncoding RNAs
(A-B) Reproducing the analyses and results presented in Fig. 1A, B, D, E usingHEK293 (A) cell or mouse embryonic stem cell (B) data (p-values represent a two-sided Wilcoxon test). The center lines of the boxplots show the medians, interquartile limits indicate the 25th and 75th percentiles, whiskers denote the farthest points at a maximum of 1.5 times the interquartile range). (C) Scatter plot showing the number of lncRNAs required to identify increased CV2 compared to expression-matched mRNAs. The x-axis represents the number of lncRNAs used for CV2 quantification. For each number of lncRNAs analyzed, expression-matched mRNAs were randomly selected followed by subsampling one expression-matched mRNA for each lncRNA (1,000 permutations, similar as in Fig. 1F,). The y-axis represents the outcome of the permutation test (median(CV2lncRNA) > median(CV2mRNA)). Grey points represent individual p-values for the permutation test. Blue points represent the 50th percentile of permutations reaching significance. Red points represent the 95th percentile of permutations reaching significance. The black dashed line represents subsampling 34 lncRNAs.
