Fig. 4: Inference of the approximate spatial locations of cell states during mouse gastrulation.
From: Systematic reconstruction of cellular trajectories across mouse embryogenesis
a, Inference of cell-type contributors to each spatial territory of the gastrulating mouse embryo based on the application of CIBERSORTx to GEO-seq data48,49. GEO-seq yields bulk RNA-sequencing data from small numbers of cells dissected from precise anatomic regions of the gastrulating embryo48. We then estimated the proportional contribution of each cell state to each GEO-seq sample using CIBERSORTx (ref. 49). b, Corn plots48 showing the spatial pattern of inferred contributions of various ectodermal cell types at E7.5. c, Corn plots showing the spatial pattern of inferred contributions of various mesodermal cell types at E7.5. d, Corn plots showing the spatial pattern of inferred contributions of various endodermal cell types at E7.5, as well as notochord. In each corn plot, each circle or diamond refers to a GEO-seq sample and its weighted color to the estimated cell-type composition. Corn plot nomenclature from Peng et al.48. A, anterior; P, posterior; L, left lateral; R, right lateral; L1, anterior left lateral; R1, anterior right lateral; L2, posterior left lateral; R2, posterior right lateral; Epi1 and Epi2, divided epiblast; M, whole mesoderm; MA, anterior mesoderm; MP, posterior mesoderm; En1 and En2, divided endoderm; EA, anterior endoderm; EP, posterior endoderm.
